Combinatorial biochemistry and shuffling of TEM, SHV and Streptomyces albus omega loops in PSE-4 class A beta-lactamase.

نویسندگان

  • F Sanschagrin
  • E Thériault
  • Y Sabbagh
  • N Voyer
  • R C Levesque
چکیده

The class A PSE-4 beta-lactamase was used for studying the importance of amino acids in the omega (Omega) loop and its interactions for hydrolysis of beta-lactam antibiotics. By cassette mutagenesis, we replaced the amino acids 163-179 Omega loop in PSE-4 with TEM-1, SHV-1 and Streptomyces albus G beta-lactamase Omega loops. Phenotypic analysis of Escherichia coli recombinants expressing the Omega loop PSE-4 mutant enzymes gave MICs and kinetic data similar to those of wild-type PSE-4.

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عنوان ژورنال:
  • The Journal of antimicrobial chemotherapy

دوره 45 4  شماره 

صفحات  -

تاریخ انتشار 2000